Journal: Experimental and Therapeutic Medicine
Article Title: New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins
doi: 10.3892/etm.2024.12520
Figure Lengend Snippet: Uterine leiomyosarcoma SKN cells were cultured in CytoSelect 48-well Cell Adhesion Assay plates, SFRP4 was added at a concentration of 2.5 µg/ml, and the absorbance was measured. The ‘0’ group was the SFRP4 untreated control group. (A) Adhesion ability of the leiomyosarcoma cells was significantly increased to each cell adhesion molecule (n=4). (B) A histological image comparing SFRP4-untreated cells and SFRP-treated (2.5 µg/ml) cells. An increase in cell number was observed in the treated group compared with the untreated group. Magnification, x40. *P<0.01 vs. control. Col, collagen; Lam, laminin I; SFRP, secreted frizzled-related protein.
Article Snippet: Similarly, to assess changes in cell adhesion, uterine leiomyosarcoma cells were seeded at a density of 5x10 2 cells/well in a CytoSelect 48-well Cell Adhesion Assay plate (cat. no. CBA-070; Cell Biolabs, Inc.) and divided into a group treated with SFRP4 at a concentration of 2.5 µg/ml at 37˚C for 24 h and a control group that received no treatment.
Techniques: Cell Culture, Cell Adhesion Assay, Concentration Assay